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Details : C10RMP10 - NCCL HPLC Reference procedure for serum Cholesterol

Type (acronym)rmp
Typereference measurement procedure
Unique Nomination NumberC10RMP10
Analyte Nametotal cholesterol
IUPAC IFCC NumberCAS57-88-5
Method capable of Traceability of Analyte to SI or Defined ProcedureSI
Applicable Matriceslyophilized, fresh, or frozen human serum
NameNCCL HPLC Reference procedure for serum Cholesterol
Organization that developed Validated MethodBeijing Hospital Institute of Geriatrics and National Center for Clinical Laboratories(NCCL)
Measurement Techniques UsedHPLC
Comments 1Oxidized to cholest-4-en-3,6-diones with chromic acid
Comments 2Calibration with NIM(National Institute of Metrology P. R. China)CRM GBW09203b Cholesterol
QuantityAmount-of-substance concentration
From (0)1.29
To (0)10.34
Unit (0)mmol/L
From (1)0.3
To (1)0.9
Unit (1)%
Level of Confidence (%)95%
Peer Reviewed PublicationJones oxidation and high performance liquid chromatographic analysis of cholesterol in biological samples, Chen W et al., J. Chromatogr. B, 2007, 858, 239-246.
Review Team Namemetabolites and substrates
Analyte Category NameMetabolites and substrates
Non-CCQM Key Comparison ReportIFCC External Quality assessment scheme for Reference Laboratories in Laboratory Medicine (RELA)
Non-CCQM Key Comparison Report Hyperlinkhttp://www.dgkl-rfb.de:81/
Non-CCQM Key Comparison Report
Labcode: 18
Non-CCQM Key Comparison Report
Results for year 2003
Measurement principle/techniqueHigh performance liquid chromatography