Accurate results
for patient care
Laboratory medicine and in vitro diagnostics
JCTLM Database: higher-order reference materials, methods and services
Details : C12RMP2 - CDC reference measurement procedure for serum 25-hydroxyvitamin D metabolites (25-hydroxyvitamin D3)
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C12RMP2 |
| Analyte Name | 25-hydroxyvitamin D3 |
| IUPAC IFCC Number | CAS63283-36-3 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | fresh, lyophilized, or frozen human serum |
| Name | CDC reference measurement procedure for serum 25-hydroxyvitamin D metabolites (25-hydroxyvitamin D3) |
| Organization that developed Validated Method | Centers for Desease Control and Prevention (CDC) |
| Measurement Techniques Used | Isotope dilution liquid chromatography mass spectrometry (ID-LC-MS/MS) |
| Comments 1 | Calibration solution tracable to SRM 2972a, 25-hydroxyvitamin D3 |
| Quantity | Amount-of-substance concentration |
| From (0) | 10 |
| To (0) | 100 |
| Unit (0) | nmol/L |
| From (1) | 2.7 |
| To (1) | 3.9 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | A candidate reference measurement procedure for quantifying serum concentrations of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 using isotope-dilution liquid chromatography-tandem mass spectrometry, Mineva E M et al., Anal. Bioanal. Chem., 2015, 407(19), 5615-5624 |
| Review Team Name | vitamins |
| Analyte Category Name | Vitamins and micronutrients |
| Measurement principle/technique | Isotope dilution mass spectrometry |