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Details : C15RMP11R - NIB reference method for Human cytomegalovirus quantification by dPCR
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C15RMP11R |
| Analyte Name | Human cytomegalovirus (HCMV) DNA sequence |
| IUPAC IFCC Number | HCMV |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | Purified gDNA in solution Water, buffer |
| Name | NIB reference method for Human cytomegalovirus quantification by dPCR |
| Organization that developed Validated Method | National institute of biology (NIB) |
| Measurement Techniques Used | Digital PCR |
| Quantity | Copy number concentration quantity to 1 |
| From (0) | 11 |
| To (0) | 38000 |
| Unit (0) | copies/20µL reaction |
| From (1) | 6 |
| To (1) | 25 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Digital PCR for direct quantification of viruses without DNA extraction, Pavsic J. et al., Anal Bioanal Chem. 2016, 408, 67-75 Assessment of the real-time PCR and different digital PCR platforms for DNA quantification, Pavsic J. et al., Anal Bioanal Chem. 2016, 408, 107-121 Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA,Pavsic J. et al., Anal Bioanal Chem. 2017, 409, 2601-2614 |
| Analyte Category Name | Nucleic acids |
| Non-CCQM Key Comparison Report | See Anal Bioanal Chem. 2017, 409, 2601 for comparability assessment study |
| Non-CCQM Key Comparison Report Hyperlink | https://doi.org/10.1007/s00216-017-0206-0 |
| Measurement principle/technique | Digital PCR |