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Details : C16RMP1 - PTB reference method for blood cell counting, erythrocyte
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C16RMP1 |
| Analyte Name | erythrocyte |
| IUPAC IFCC Number | erythrocyte |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | fresh or stabilized whole blood |
| Name | PTB reference method for blood cell counting, erythrocyte |
| Organization that developed Validated Method | Physikalisch-Technische Bundesanstalt (PTB) |
| Measurement Techniques Used | Flow cytometry, cell differentiation by impedance or light scattering, coincidence correction by dilution series |
| Quantity | Particle concentration |
| From (0) | 0.5 |
| To (0) | 7 |
| Unit (0) | (1/pl) |
| From (1) | 1.5 |
| To (1) | 1.5 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | German National Standard DIN 58932-3: 2017-01 (German and English) More about coincidence loss and reference methods, Helleman P.W., Phys. Med. Biol., 1990, 35, 1159-1162 P.W. Helleman, PhD Thesis, De Bilt Netherlands 1972, 185-255 (Chapter 9 - 11) Reference measurement procedures for the accurate determination of cell concentrations: present status and future developments, Kammel M. et al, J. Lab. Medicine, 2012, 36, 25-35 |
| How to Obtain Method Copy | Contact DIN e-mail: joerg.neukammer@ptb.de, andreas.kummrow@ptb.de |
| Multiple Testing Sites Not Required to obtain a Single Value | Multiple testing sites not required |
| Additional Comments | validation includes the determination of reference values by instuments using different methods of cell differentiation (i.e. impedance change / light scatter). In addition, instruments which do not use hydrodynamic focussing are included in the study |
| Comments to be published via JCTLM DB | Coincidence corrrection by linear extrapolation as described by Lewis et al. (Phys. Med. Biol., 1989, 34, 1239-1246) and England et al. (Clin. Lab. Haemat., 1994, 16, 131-138) cannot be used to determine reference values (see Helleman 1990). Analysis of dilution series as described by P.W. Helleman 1972 and in DIN 58932-3, DIN 58932-4, and DIN 58932-5 includes intrumental characteristics, e.g. orifice size, as well as particle properties like volume and flexibility, in contrast to the statement by Lewis et al. An extensive data analysis including the determination of uncertainties according to the "Guide to the expression of uncertainty in measurement" in given in the appendix of DIN 58932-3 |
| Analyte Category Name | Blood cell counting |
| Measurement principle/technique | Flow cytometry |