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Details : C16RMP2R - UPLC-tandem mass spectrometry method for analysis of amyloid beta 1-40 in human CSF
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C16RMP2R |
| Analyte Name | amyloid beta 1-40 |
| IUPAC IFCC Number | Amyloid beta 1-40 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | human CSF, frozen |
| Name | UPLC-tandem mass spectrometry method for analysis of amyloid beta 1-40 in human CSF |
| Organization that developed Validated Method | Biomarker Research Laboratory, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA |
| Measurement Techniques Used | liquid chromatography tandem mass spectrometry, solid phase extraction |
| Quantity | Mass concentration |
| From (0) | 200 |
| To (0) | 20000 |
| Unit (0) | pg/mL |
| From (1) | 17.4 |
| To (1) | 1085.6 |
| Unit (1) | pg/mL |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Qualification of a surrogate matrix-based absolute quantification method for Amyloid-beta 42 in human cerebrospinal fluid using 2D UPLC-tandem mass spectrometry, Korecka M. et al, ''Journal of Alzheimer's Disease, 2014, 41(2), 441-451 Analytical and clinical performance of Amyloid-beta peptides measurements in CSF of ADNIGO/2 participants by an LC-MS/MS Reference Method, Korecka M. et al, Clinical Chemistry, 2020, 66(4), 587-597 Analytical and clinical performance of amyloid beta peptides measurement in CSF of ADNIGO and ADNI2 participants by an UPLC-MS-MS reference method, Korecka M., Poster for 2018 AAIC, Alzheimer's and Dementia'', 2018, 14(7), 378 |
| Comments to be published via JCTLM DB | This reference measurement method was developed and validated by Biomarker Research Laboratory, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA |
| Analyte Category Name | Proteins |
| Non-CCQM Key Comparison Report | Commutability study - Amyloid beta 42 adjusted methods- this project focused not only on comparison of Ab42 between immunoassays: Euroimmune, Fujirebio, Roche and mass spec (UPenn) after adjustment to Ab42 CRMs, but also included analysis of Abeta 40. Data are still under review so official or published report is not available. |
| Non-CCQM Key Comparison Report Hyperlink | |
| Measurement principle/technique | Liquid chromatography mass spectrometry |