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Details : C19RMP11 - ID-LC-MS/MS-based reference measurement procedure for the quantification of methotrexate in human serum and plasma
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C19RMP11 |
| Analyte Name | methotrexate |
| IUPAC IFCC Number | CAS59-05-2 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | yes |
| Applicable Matrices | human serum and plasma; fresh or frozen Lithium heparin plasma, K3-EDTA plasma, K2-EDTA plasma |
| Name | ID-LC-MS/MS-based reference measurement procedure for the quantification of methotrexate in human serum and plasma |
| Organization that developed Validated Method | Labor Berlin - Charité Vivantes Services GmbH in collaboration with Roche Diagnostics GmbH |
| Measurement Techniques Used | Isotope dilution mass spectrometry |
| Quantity | Mass concentration |
| From (0) | 7.2 |
| To (0) | 5700 |
| Unit (0) | ng/mL |
| From (1) | 7.8 |
| To (1) | 3.6 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Comments on Uncertainty Range | The lowest expected measurement uncertainty of 7.8 % is at the mass concentration of 24.00 ng/mL. The highest expected measurement uncertainty of 3.6 % is at the mass concentration of 4200 ng/mL. |
| Peer Reviewed Publication | An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of methotrexate in human serum and plasma, Taibon J. et al, Clinical Chemisty and Laboratory Medicine 2023 61(11) 1917-1929 |
| Comments to be published via JCTLM DB | This reference measurement procedure was developed and validated by Labor Berlin - Charité Vivantes Services GmbH in collaboration with Roche Diagnostics GmbH |
| Analyte Category Name | Drugs |
| Non-CCQM Key Comparison Report | See CCLM publication cited above for comparability assessment study |
| Measurement principle/technique | Isotope dilution mass spectrometry |