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Details : C1RMP_P16_MS - IFCC method for HbA1c (HPLC / MS-ESI)
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C1RMP_P16_MS |
| Analyte Name | HbA1c |
| IUPAC IFCC Number | QU60433 |
| Applicable Matrices | whole blood |
| Name | IFCC method for HbA1c (HPLC / MS-ESI) |
| Organization that developed Validated Method | IFCC |
| Measurement Techniques Used | Hemoglobin samples treated with endoproteinase Glu-C followed by HPLC and electrospray ionisation mass spectrometry (ESI-MS) |
| Quantity | Amount-of-substance fraction of [HbA1c/(HbA1c+HbA0)] expressed in mmol/mol |
| From (0) | 0 |
| To (0) | 140 |
| Unit (0) | mmol/mol |
| From (1) | 1 |
| To (1) | 1 |
| Unit (1) | % |
| Peer Reviewed Publication | Kaiser P. et al., Modification of the IFCC reference measurement procedure for determination of HbA1c by HPLC-ESI-MS, GMS Ger. Med. Sci., 2006, Doc. 06. Jeppsson J-O et al., Approved IFCC reference method for the measurement of HbA1c in human blood, Clin. Chem. Lab. Med., 2002, 40, 78-89 A modification of the method as described in Clin. Chem., 2008, 54, 1018-22 has been accepted by the IFCC network and is currently used by the IFCC network Laboratories. |
| Review Team Name | proteins |
| Analyte Category Name | Proteins |
| Non-CCQM Key Comparison Report | There are three proposed reference measurement procedures listed for HbA1c. One, based on chromatography has been the reference used for a number of years. Two more recent procedures (endorsed by the IFCC) more rigorously determine the amount-of-substance ratio of HbA1c and have been proposed to replace earlier method. The methods have been demonstrated to correlate (precisely) with one another but result in different reference values and limits. While the IFCC recommended procedures might be considered higher methods relative to the chromatographic method, all are higher order to a manufacturer's method. The Master Conversion Equation for relation between the IFCC numbers and the respective national Designated Comparisons Methods was published in Clinical Chemistry, 2004, 50, N°1, 166-174. |
| Non-CCQM Key Comparison Report Hyperlink | |
| Measurement principle/technique | High performance liquid chromatography |