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Details : C1RMP_P17 - Reference method for diabetes control and complications trial
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C1RMP_P17 |
| Analyte Name | HbA1c |
| IUPAC IFCC Number | QU60433 |
| Applicable Matrices | whole blood |
| Name | Reference method for diabetes control and complications trial |
| Organization that developed Validated Method | DCCT |
| Measurement Techniques Used | Cation-exchange HPLC using Bio-Rex 70 resin |
| Quantity | Chomatographic peak area fraction of HbA1c |
| From (0) | 4 |
| To (0) | 11 |
| Unit (0) | % |
| From (1) | 2 |
| To (1) | 2 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Goldstein et al., Measurement of glycosylated hemoglobin: High performance liquid chromatographic and thiobarbituric acid colorimetric methods. In: Methods in diabetes research, Volume II: Clinical Methods, Clarke WL, Larner J and Pohl SL, eds. NY: Wiley and Sons, Inc: 475-504, 1986 |
| Review Team Name | proteins |
| Analyte Category Name | Proteins |
| Non-CCQM Key Comparison Report | There are three proposed reference measurement procedures listed for HbA1c. One, based on chromatography has been the reference used for a number of years. Two more recent procedures (endorsed by the IFCC) more rigorously determine the amount-of-substance ratio of HbA1c and have been proposed to replace earlier method. The methods have been demonstrated to correlate (precisely) with one another but result in different reference values and limits. While the IFCC recommended procedures might be considered higher methods relative to the chromatographic method, all are higher order to a manufacturer's method. The Master Conversion Equation for relation between the IFCC numbers and the respective national Designated Comparisons Methods was published in Clinical Chemistry, 2004, 50, N°1, 166-174. |
| Non-CCQM Key Comparison Report Hyperlink | |
| Measurement principle/technique | High performance liquid chromatography |