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Details : C20RMP3 - PTB reference method for human immunodeficiency virus type 1 (HIV-1) quantification by RT-dPCR
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C20RMP3 |
| Analyte Name | Human immunodeficiency virus type 1 (HIV-1) RNA sequence |
| Method capable of Traceability of Analyte to SI or Defined Procedure | Traceability to defined procedure (See references) |
| Applicable Matrices | RNA solution |
| Name | PTB reference method for human immunodeficiency virus type 1 (HIV-1) quantification by RT-dPCR |
| Organization that developed Validated Method | Physikalisch-Technische Bundesanstalt (PTB) |
| Measurement Techniques Used | Digital PCR |
| Quantity | RNA copy number concentration |
| From (0) | 100 |
| To (0) | 10000 |
| Unit (0) | copies/µL |
| From (1) | 25 |
| To (1) | 28 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Ref. 1 - An assessment of the reproducibility of reverse transcription digital PCR quantification of HIV-1, Falak S. et al, Methods 2022 201 34-40 Ref. 2 - CCQM-P199: Interlaboratory comparability study of HIV-1 RNA copy number quantification DOI: 2024.04.12.589042 |
| Comments to be published via JCTLM DB | Additionnal information on the RMP is available from the following LINK to provide further detailed information on the following points:
|
| Analyte Category Name | Nucleic acids |
| CCQM Key Comparison Report | See above references for comparability studies |
| Measurement principle/technique | Digital PCR |