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Details : C20RMP9 - ID-LC-MS/MS-based reference measurement procedure for the quantification of phenobarbital in human serum and plasma
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | C20RMP9 |
| Analyte Name | phenobarbital |
| IUPAC IFCC Number | CAS50-06-6 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | yes |
| Applicable Matrices | human serum and plasma; fresh or frozen |
| Name | ID-LC-MS/MS-based reference measurement procedure for the quantification of phenobarbital in human serum and plasma |
| Organization that developed Validated Method | This reference measurement procedure was developed and validated by the laboratory of Dr. Risch Ostschweiz AG in Buchs SG, Switzerland in collaboration with Roche Diagnostics GmbH in Penzberg, Germany |
| Measurement Techniques Used | Isotope dilution mass spectrometry |
| Quantity | Mass concentration |
| From (0) | 1.92 |
| To (0) | 72 |
| Unit (0) | µg/mL |
| From (1) | 3.3 |
| To (1) | 2.1 |
| Unit (1) | % |
| Level of Confidence (%) | 0.95 |
| Comments on Uncertainty Range | The expected measurement uncertainty of 3.3 % is at the mass concentration of 2.00 µg/mL, where n = 6, sample preparations. The expected measurement uncertainty of 2.1 % is at the mass concentration of 50.0 µg/mL, , where n = 6, sample preparations. |
| Peer Reviewed Publication | An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of phenobarbital in human serum and plasma, Taibon J. et al, Clinical Chemisty and Laboratory Medicine 2024 62(7) 1314-1326 |
| Comments to be published via JCTLM DB | This reference measurement procedure was developed and validated by the laboratory of Dr. Risch Ostschweiz AG in Buchs SG, Switzerland in collaboration with Roche Diagnostics GmbH in Penzberg, Germany |
| Analyte Category Name | Drugs |
| Non-CCQM Key Comparison Report | A method comparison with the existing JCTLM listed method is not possible, as the method is not in current use at NIST and there are no plans to reinstitute this RMP at NIST in the foreseeable future. |
| Non-CCQM Key Comparison Report | See CCLM publication cited above for comparability assessment study between the two laboratories having developed and validated the method. |
| Measurement principle/technique | Isotope dilution mass spectrometry |