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Details : CYC_II_L_I_RMP_04 - CDC reference method for HDL cholesterol in serum
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | CYC_II_L_I_RMP_04 |
| Analyte Name | HDL cholesterol |
| IUPAC IFCC Number | HDL cholesterol |
| Method capable of Traceability of Analyte to SI or Defined Procedure | procedurally-defined |
| Applicable Matrices | fresh or frozen serum |
| Name | CDC reference method for HDL cholesterol in serum |
| Organization that developed Validated Method | CDC, Atlanta, GA, USA |
| Measurement Techniques Used | Ultracentrifugation removes VLDL, d < 1.006, preventing interference (TG) with the heparin/Mn2+ precipitation step for removal of non-HDL. HDL cholesterol is spectrophotometric. |
| Comments 1 | HDL cholesterol in heparin/Mn2+ supernatant measured by CDC Abell-Kendall cholesterol reference method which is calibrated with SRM 911b, high purity cholesterol |
| Comments 2 | This is a procedurally-defined method that uses SI units for cholesterol. Defined density , UC speed, temperatures, regularly verified. |
| Quantity | Amount-of-substance concentration |
| From (0) | 0.6 |
| To (0) | 2.6 |
| Unit (0) | mmol/l |
| From (1) | 1.2 |
| To (1) | 0.7 |
| Unit (1) | % |
| Peer Reviewed Publication | In: Handbook of Lipoprotein Testing, 2nd Edition. Washington DC: AACC Press 2001: 227-230. |
| How to Obtain Method Copy | Contact Parvin Waymack, pwaymack@cdc.gov for detailed version. HDL method issues described: Clin. Chem., 2002, 48, A110; Clin. Chem., 2002, 48, A109; Clin. Chem., 2001, 47, A58. |
| Additional Comments | Used to value-assign CDC reference materials used in the CDC-NHLBI Lipid Standardization Program and to standardize CDC-CRMLN HDLC designated comparison method, Clin Chem 45, 1803-12 (1999); The % reative expanded unceratinty was calculated from QC data and value -assignment data from the last 10 years. |
| Review Team Name | metabolites and substrates |
| Analyte Category Name | Metabolites and substrates |
| Measurement principle/technique | Ultracentrifugation spectrophotometry |