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Details : CYC_II_L_I_RMP_05 - CDC beta-quantification reference method for LDL cholesterol in serum, (extension of CDC HDL cholesterol method)
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | CYC_II_L_I_RMP_05 |
| Analyte Name | LDL cholesterol |
| IUPAC IFCC Number | LDL cholesterol |
| Method capable of Traceability of Analyte to SI or Defined Procedure | procedurally-defined |
| Applicable Matrices | fresh, or frozen serum |
| Name | CDC beta-quantification reference method for LDL cholesterol in serum, (extension of CDC HDL cholesterol method) |
| Organization that developed Validated Method | CDC, Atlanta, GA, USA |
| Measurement Techniques Used | Ultracentrifugation removes VLDL, d < 1.006, preventing interference (TG) with the heparin/Mn2+ precipitation step for removal of non-HDL. HDLC quantitation is spectrophotometric. |
| Comments 1 | d>1.006 bottom fraction cholesterol measured by CDC Abell-Kendall cholesterol reference method; LDLC = BFC -HDLC; [LDLC defined by NCEP includes IDLC and Lp(a)C.] |
| Comments 2 | Extends HDLC method by using d>1.006 bottom fraction from same UC samples of the CDC HDL cholesterol reference method |
| Quantity | Amount-of-substance concentration |
| From (0) | 1.3 |
| To (0) | 9.7 |
| Unit (0) | mmol/l |
| From (1) | 1.3 |
| To (1) | 1 |
| Unit (1) | % |
| Peer Reviewed Publication | In: Handbook of Lipoprotein Testing, 2nd Edition. Washington DC: AACC Press 2001: 227-230. |
| How to Obtain Method Copy | Contact Parvin Waymack, pwaymack@cdc.gov for detailed version. LDL method issues described: Clin. Chem., 2003, 49, A110; Clin. Chem., 2000, 46, A109; Clin. Chem., 1996, 42, A58. |
| Additional Comments | Used to standardize CDC-CRMLN LDLC. % reative expanded unceratinty was calculated from QC data and value-assignment data from the last 7 years |
| Review Team Name | metabolites and substrates |
| Analyte Category Name | Metabolites and substrates |
| Measurement principle/technique | Ultracentrifugation spectrophotometry |