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Details : NRMeth 10 - University of Ghent reference measurement procedure for estradiol

Type (acronym)rmp
Typereference measurement procedure
Unique Nomination NumberNRMeth 10
Analyte Name17β-estradiol
IUPAC IFCC NumberCAS50-28-2
Method capable of Traceability of Analyte to SI or Defined ProcedureSI
Applicable Matriceslyophilized, fresh, or frozen human serum
NameUniversity of Ghent reference measurement procedure for estradiol
Organization that developed Validated MethodUniversity of Ghent
Measurement Techniques UsedID/GC/MS
Comments 1Heptafluorobutyryl derivative
Comments 2Calibrated with Merck AG high purity estradiol; accuracy control with CRM 576-578 and DGKC-sera
QuantityAmount-of-substance concentration
From (0)0.015
To (0)30
Unit (0)nmol/l
From (1)1.5
To (1)3
Unit (1)%
Level of Confidence (%)95%
Peer Reviewed PublicationClin. Chem., 1988, 34, 2066-2069 Eur. J. Clin. Chem. Clin. Biochem., 1996, 34, 853-860 Clin. Chem., 1998, 44, 671-674
Review Team Namenon-peptide hormones
Analyte Category NameNon-peptide hormones
Non-CCQM Key Comparison ReportThe certification of estradiol-17b in three lyophilized serum materials CRM 576, CRM 577 and CRM 578: Report EUR 17540 EN, 1997
Non-CCQM Key Comparison Report Hyperlink
Measurement principle/techniqueIsotope dilution mass spectrometry