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Details : NRMeth 29 - Doumas reference method for total bilirubin
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | NRMeth 29 |
| Analyte Name | bilirubin |
| IUPAC IFCC Number | CAS635-65-4 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | lyophillized, fresh or frozen human serum |
| Name | Doumas reference method for total bilirubin |
| Organization that developed Validated Method | NCCLS / NRSCL |
| Measurement Techniques Used | Spectrophotometry |
| Comments 1 | diazo method (Jendrassik-Grof principle) |
| Comments 2 | calibrate with SRM 916a, high purity bilirubin; Method uncertainty as CV% from interlaboratory studies based on literature. |
| Quantity | Amount-of-substance concentration |
| From (0) | 9 |
| To (0) | 43 |
| Unit (0) | µmol/l |
| From (1) | 1.5 |
| To (1) | 4 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Clin. Chem., 1985, 31, 1779-1789 Clin. Chem., 1983, 29, 297-301 |
| How to Obtain Method Copy | Contact NCCLS |
| Review Team Name | metabolites and substrates |
| Analyte Category Name | Metabolites and substrates |
| Measurement principle/technique | Spectrophotometry |