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Details : NRMeth 39 - Mayo reference method for 17-hydroxy-progesterone
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | NRMeth 39 |
| Analyte Name | 17-hydroxyprogesterone |
| IUPAC IFCC Number | CAS604-09-1 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | human serum |
| Name | Mayo reference method for 17-hydroxy-progesterone |
| Organization that developed Validated Method | Mayo Foundation |
| Measurement Techniques Used | LC/MS/MS |
| Comments 1 | Ion Pair 331.20/1109.1 normalized to d8-17OHPG ion pair 339.0/112.9 |
| Comments 2 | C18 column (Supleco Supelcosil) |
| Quantity | Amount-of-substance concentration |
| From (0) | 320 |
| To (0) | 302000 |
| Unit (0) | pmol/l |
| Peer Reviewed Publication | Machacek D. A., Singh R. J., Development and validation of serum 17 alpha-hydroxy progesterone LC-MS/MS assay for screening and monitoring of congenital adrenal hyperplasia, presented at American Society of Mass Spectrometry meeting, 2001, Chicago Method based on: Wudy S. A., Hartmann M., Svoboda M., Determination of 17-hydroxyprogesterone in plasma by stable isotope dilution/benchtop liquid chromatography-tandem mass spectrometry, Horm. Res., 2000, 53, 68-71 |
| Additional Comments | Clinical method currently being expanded to ref method |
| Review Team Name | non-peptide hormones |
| Analyte Category Name | Non-peptide hormones |
| Measurement principle/technique | Liquid chromatography mass spectrometry |