JCTLM Database: higher-order reference materials, methods and services
Details : NRMeth 5 - University of Ghent reference measurement procedure for total glycerol
| Type (acronym) | rmp |
| Type | reference measurement procedure |
| Unique Nomination Number | NRMeth 5 |
| Analyte Name | total glycerol |
| IUPAC IFCC Number | total glycerol |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | lyophilized, fresh, or frozen human serum |
| Name | University of Ghent reference measurement procedure for total glycerol |
| Organization that developed Validated Method | University of Ghent |
| Measurement Techniques Used | ID/GC/MS |
| Comments 1 | Heptafluorobutyryl derivative |
| Comments 2 | Calibrated with SRM 1595, high purity tripalmitin; accuracy control with SRM 909b and DGKC-sera |
| Quantity | Amount-of-substance concentration |
| From (0) | 0.15 |
| To (0) | 5 |
| Unit (0) | mmol/l |
| From (1) | 1 |
| To (1) | 2 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Eur. J. Clin. Chem. Clin. Biochem., 1996, 34, 853-860
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| Comments to be published via JCTLM DB | This reference measurement procedure measures 'total glycerol' in serum, which is the sum of triglycerides, diglycerides, monoglycerides, and free glycerol. |
| Review Team Name | metabolites and substrates |
| Analyte Category Name | Metabolites and substrates |
| Measurement principle/technique | Isotope dilution mass spectrometry |