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Details : NRMeth 57 - DGKC reference method for serum cortisol
| Type (acronym) | rmp |
|---|---|
| Type | reference measurement procedure |
| Unique Nomination Number | NRMeth 57 |
| Analyte Name | cortisol |
| IUPAC IFCC Number | CAS50-23-7 |
| Method capable of Traceability of Analyte to SI or Defined Procedure | SI |
| Applicable Matrices | lyophilized, fresh, or frozen human serum or plasma |
| Name | DGKC reference method for serum cortisol |
| Organization that developed Validated Method | German Society of Clinical Chemistry (DGKC) |
| Measurement Techniques Used | ID/HPLC/MS and ID/GC/MS |
| Comments 1 | Methoxime - Trimehylsilyl ether (ID/GC/MS) |
| Comments 2 | Calibrate with SRM 921 , high purity cortisol |
| Quantity | Amount-of-substance concentration |
| From (0) | 40 |
| To (0) | 1500 |
| Unit (0) | nmol/l |
| From (1) | 1 |
| To (1) | 3 |
| Unit (1) | % |
| Level of Confidence (%) | 95% |
| Peer Reviewed Publication | Siekmann et al., J. Clin. Chem. Clin. Biochem., 1982, 20, 883-892 |
| How to Obtain Method Copy | e-mail lothar.siekmann@ukb.uni-bonn.de |
| Review Team Name | non-peptide hormones |
| Analyte Category Name | Non-peptide hormones |
| Non-CCQM Key Comparison Report | Certification of cortisol in two lyophilised serum materials BCR CRM 192 and 193: Report EUR 9661 EN, 1985 |
| Non-CCQM Key Comparison Report Hyperlink | |
| Measurement principle/technique | Isotope dilution mass spectrometry |