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Details : NRMeth40_Estradiol - Mayo reference measurement method for 17β-estradiol in blood plasma

Type (acronym)rmp
Typereference measurement procedure
Unique Nomination NumberNRMeth40_Estradiol
Analyte Name17β-estradiol
IUPAC IFCC NumberCAS50-28-2
Method capable of Traceability of Analyte to SI or Defined ProcedureSI
Applicable Matriceshuman plasma
NameMayo reference measurement method for 17β-estradiol in blood plasma
Organization that developed Validated MethodMayo Foundation
Measurement Techniques UsedLC-MS/MS
Comments 1Ion Pair 271/145 normalized to d5-estradiol ion pair 276/187
Comments 2C18 column (Supleco Supelcosil)
QuantityAmount-of-substance concentration
From (0)0.037
To (0)7.4
Unit (0)nmol/l
Peer Reviewed PublicationLiquid Chromatography Tandem Mass Spectrometry Assay for Simultaneous Measurement of Estradiol and Estrone in Human Plasma, Nelson R et al., Clin. Chem., 2004, 50, 373
Review Team Namenon-peptide hormones
Analyte Category NameNon-peptide hormones
Measurement principle/techniqueLiquid chromatography mass spectrometry